Internal Standard - Wikipedia
An internal standard in analytical chemistry is a chemical substance is an internal standard used in the analysis of volatiles on GC-MS because it is similar to The internal standard selected should be again similar to the analyte and have a similar retention time and similar ... Read Article
The Agilent 7890A Gas Chromatograph - George Mason
The Agilent 7890A Gas Chromatograph All the elements for perfect chemistry. Our measure is your success. digital electronics set a new benchmark for retention time locking (RTL) precision and help make the 7890A Agilent’s most dependable GC ever. ... Content Retrieval
Retention Time Changes - Alfresco.ubm-us.net
2 LC•GC Europe 18(1) 12–16 (2005). LC TROUBLESHOOTING The retention time of a peak in a liquid chromatography (LC) separation can be a useful diagnostic tool to identify problems Changes in retention time can be a key to tracking down LC problems. ... Access Document
HPLC Troubleshooting - Proteins And Proteomics
HPLC Troubleshooting 1. INTRODUCTION Locating and Correcting the Problem Prevention 2. ABNORMAL PRESSURE A. No Pressure Reading,No Flow B. No Pressure Reading,Flow Is Normal J. Retention Time Drifts K. Abrupt Retention Time Changes L. Baseline Drift M. Baseline Noise (Regular) ... Visit Document
Basic Operations Guide To The Analysis Of Sulfur Using The ...
1) Run a standard through the GC in order to determine the correct retention time for mustard (refer to section 3.1.1). Ensure that most recent method is used. 2) Click on the ‘View/Edit Methods’ button (second from left in Star toolbar). 3) Click ‘File’ then ‘Open an Existing Method’. ... View This Document
GC Troubleshooting 10 - CHROMacademy
Poor sensitivity with increased retention time Poor sensitivity with normal retention time Retention times decreasing Retention times increasing Low reproducibility of retention times Retention times are inconsistent See also: Results related problems with specific system components Back to top level troubleshooting list. ... Get Content Here
Troubleshooting And Diagnostics Tips And Tricks - Waters
Troubleshooting and diagnostics Troubleshooting –System Pressure Problems –Incorrect Retention time –Loss of precision –Carryover/Contamination ... Get Content Here
LC Troubleshooting Series Retention Time Shifts - Agilent
LC Troubleshooting Series . Retention Time Shifts . Introduction . John Henderson is an Applications Scientist for Agilent Chemistries and Supplies Division. Maureen Joseph is the HPLC Columns Product Manager. Some possible causes to consider for retention time changes are: • Column aging • Column contamination ... View Document
LC Chromatography Troubleshooting - LCGCINDIA
LC Chromatography Troubleshooting Make sure elution time is sufficient. It may be necessary to flush the column at the end of run In this case you will typically see the peak retention time shift slightly shorter. This is also the simplest to ... Read Here
HPLC Troubleshooting Cover - University Of Pittsburgh
HPLC Troubleshooting Uwe D. Neue, Waters Corporation 1. Column Life-Time 2. Variable Retention Times 3. Drifting Retention Times change of as little as 0.1 pH units can result in a retention time shift of 10%. So it is very important to measure the pH accurately and to keep the pH meter well ... Retrieve Content
Successful HPLC Operation - Troubleshooting Guide
A Troubleshooting Guide – Version 1.1. 2 1. Introduction 4 1.1 Purpose 4 3.4.3 Increasing or Decreasing to a New Constant Retention Time 17 Each troubleshooting chapter contains information on the most likely cause(s) of the observed ... Fetch Content
GC And GCMS Fundamentals Of Instrument Troubleshooting And ...
GC and GCMS Fundamentals of Instrument Troubleshooting and Maintenance August 1, 2016 Kirk Lokits Ph.D. Applications Chemist. 1971 5930A with Oscilloscope and Strip Chart Retention Time Shift 2.00 3.25 4.75 2.75 4.00 5.50 INJECTOR-Leak in the septum ... Retrieve Full Source
Everything Was Just Fine And Then This Happened!”
Troubleshooting Techniques, Tips, and Tricks Daron Decker Chromatography Technical Specialist Page 2 “Everything was just fine and then Retention Time Shift 2.00 3.25 4.75 2.75 4.00 5.50 INJECTOR-Leak in the septum-Change in injection solvent ... Access Full Source
Retention Time Vs Peak Area - Waters Corporation
From 0.995 - 1.005 ml/min in order to imitate poor retention time reproducibility. This experiment was used to calculate the effects of retention time reproducibility on peak area reproducibility. Retention Time Vs Peak Area What is the relationship? Waters 2690 Separation Module Symmetry C18 3.9 x 150mm 1 mL/min, 60:40 ... Fetch This Document
GC Troubleshooting Poster - Restek.com
• Extend GC oven equilibration time. • Use autosampler or standardize manual injection procedure. Causes • Reduce retention of compounds by This handy GC troubleshooting poster summarizes the basic steps to fast, effective troubleshooting of GC instruments and analyses. ... Access This Document
GC - Phenova.com
INDEXGC Troubleshooting Guide BEFORE YOU START 03 PEAK SHAPE PROBLEMS 12 21 Retention Time Shifts 22 Solvent Peak Broad 22 Resolution Loss 23 Performance Loss Allow sufficient time for (tem-perature) equilibration of the detector or oven. ... Read More
HPLC Troubleshooting Guide - MAC-MOD
This troubleshooting guide contains examples of some of the most common problems observed in reversed-phase HPLC (RP-HPLC) separations. Four major problem areas are covered: peak shape, retention time changes, ghost peaks and problems related to column backpressure. In addition, a section on column care is ... View Doc
Practical LC-MS Troubleshooting And Maintenance: A Primer
Practical LC-MS Troubleshooting and Maintenance: A Primer Sue Abbatiello, The Broad Institute troubleshooting is at the time your instrument is installed… Compare to Installation Values •Retention time •Peak shape, FWHM •Chromatographic resolution MS LC . ... Get Document
Split/Splitless Gas Chromatography Injection - Huntsville, TX
Split/Splitless Gas Chromatography Injection Thomas G. Chasteen (similar retention times). At masses much above this the But this is time- and analyst-intensive and analytical laboratories are all about efficiency. So this is where the ... View This Document
High-performance Liquid Chromatography - Wikipedia
The retention time measured under particular conditions is an identifying characteristic of a given analyte. Gas chromatography (GC) at the time was more powerful than liquid These ultra high performance liquid chromatography" systems or UHPLCs can work at up to 120 MPa (17,405 lbf/in 2 ... Read Article
Technical Workshops Series One Day Intensive Workshop On Gas ...
GC detectors. Qualitative & Quantitative Analysis using GC. Basic maintenance of GC. Troubleshooting process for problems like contamination, ghost peaks, baseline drift, peak tailing, reduced peak size, carrier gas leakage, and retention time shift. Practical applications of GC in the Industry. ... View Full Source
No comments:
Post a Comment